CD40 Monoclonal Antibody (1C10), Super Bright 436, eBioscience eBioscience 小鼠CD40单克隆抗体Super Bright 436

2024-10-28

CD40 Monoclonal Antibody (1C10), Super Bright 436, eBioscience

货号:62-0401-82

规格:100 μg

市场价格:3104

产品类型:流式抗体

品牌:eBioscience

抗原:CD40

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a, kappa

克隆号:1C10

荧光染料:Super Bright 436

类型: 流式抗体同型对照:Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436, eBioscience
浓度: 0.2 mg/mL用法:0.5 μg/test(Flow)
产品详细信息Description:

The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据
CD40 Antibody (62-0401-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193) and 0.25 µg of Rat IgG2a K Isotype Control Super Bright 436 (Product # 62-4321) (left) or 0.25 µg of Anti-Mouse CD40 Super Bright 436 (Product # 62-0401) (right). Total viable cells were used for analysis.
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参考文献:

1.PloS oneThe nucleosome (histone-DNA complex) is the TLR9-specific immunostimulatory component of Plasmodium falciparum that activates DCs."15-0862 was used in Flow cytometry/Cell sorting to provide a significant insight into the activation of DCs by malaria parasites."2.Experimental and therapeutic medicineLung dendritic cells undergo maturation and polarization towards a T helper type 2-stimulating phenotype in a mouse model of asthma: Role of nerve growth factor."Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry"3.Nature communicationsInjectable cryogel-based whole-cell cancer vaccines."Published figure using CD86 (B7-2) monoclonal antibody (Product # 15-0862-82) in Flow Cytometry"

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