CD23 Monoclonal Antibody (B3B4), eFluor 660, eBioscience
货号:50-0232-82
规格:100 μg
市场价格:2271
产品类型:流式抗体
品牌:eBioscience
抗原:CD23
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a, kappa
克隆号:B3B4
荧光染料:eFluor 660
类型: | 流式抗体 | 同型对照: | Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience |
浓度: | 0.2 mg/mL | 用法: | 0.5 μg/test(Flow) |
产品详细信息Description:
The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.Applications Reported: This B3B4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood andtonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.
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数据 |
CD23 Antibody (50-0232-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.25 µg of Anti-Mouse CD23 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis. |
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参考文献: |
1.Frontiers in immunologyProlonged Deleterious Influences of Chemotherapeutic Agent CPT-11 on Resident Peritoneal Macrophages and B1 Cells."Published figure using CD23 monoclonal antibody (Product # 50-0232-82) in Flow Cytometry"2.Frontiers in immunologyPeritoneal Cells Mediate Immune Responses and Cross-Protection Against Influenza A Virus."Published figure using CD23 monoclonal antibody (Product # 50-0232-82) in Flow Cytometry"3.BloodA critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation."Published figure using CD23 monoclonal antibody (Product # 50-0232-82) in Flow Cytometry" |
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