货号：12-5856-82

规格：100 µg

市场价格：2677

产品类型：流式抗体

品牌：eBioscience

抗原：Ly-49A

物种：小鼠

宿主：小鼠

抗体亚型：其它

克隆号：A1 (Ly49A)

荧光染料：PE

This A1 monoclonal antibody reacts with Ly49A, a 44-kDa member of the Ly49 family of type II transmembrane receptors expressed on natural killer (NK) cells. This receptor is present as a disulfide-linked homodimer with C-type lectin extracellular domains. An inhibitory receptor, Ly49A binds MHC class I molecules, such as H-2Dd, H-2Dk, and H-2DP, to inhibit NK-mediated cytotoxicity by triggering phosphorylation of its ITIM domain and recruitment of SHP-1 and SHP-2. Ly49A is expressedon DX5+CD3- NK cells and DX5+CD3+ NK T cells, as well as on a population of memory CD8+ T cells in C57BL/6, C57BL/10, and B10 congenic mice.Applications Reported: This A1 (Ly49A) antibody has been reported for use in flow cytometric analysis.Applications Tested: This A1 (Ly49A) antibody has been tested by flow cytometric analysis on C57Bl/6 splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.

KLRA1 (also known and Ly49L) is a member of the LY49 family of receptors Natural killer (NK) cells that bind to major histocompatibility complex (MHC) class 1. These proteins are classified as either activating or inhibitory receptors based on whether they possess an immunoreceptor tyrosine-based inhibitory motif (ITIM) in their cytoplasmic region (for inhibitory receptors), or an immunoreceptor tyrosine-based activation motif (ITAM) that transmits activating signals resulting in phosphorylationof several substrates. KLRA1 is thought to be an activating receptor, inducing DAP12 phosphorylation in response to antibody-mediated cross-linking of KLRA1 on NK cells. At least three isoforms of KLRA1 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。