Ly-49A/D Monoclonal Antibody (eBio12A8 (12A8)), PE, eBioscience
货号:12-5783-81
规格:50 µg
市场价格:1132
产品类型:流式抗体
品牌:eBioscience
抗原:Ly-49A/D
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a, kappa
克隆号:eBio12A8 (12A8)
荧光染料:PE
类型: | 流式抗体 | 同型对照: | Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience |
浓度: | 0.2 mg/mL | 用法: | 0.125 μg/test(Flow) |
产品详细信息Description:
The eBio12A8 antibody reacts with Ly-49A and Ly-49D. The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.eBio12A8 does not react with BALB/c, DBA/2, AKR, or CBA/J Ly-D49D.Applications Reported: This eBio12A8 (12A8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio12A8 (12A8) antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate-recognition domains which bind to MHC class I alloantigens. The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor homodimers. Ly-49D is expressed on subsets of natural killer cells in C57BL/6, C3H/He, and SJL mice, but not DBA/2, AKR, CBA/J, or BALB/c mice. The Ly-49D antigen has not been detected on NK-1.1+ T cells. The Ly-49D receptor has been implicated in mediation of NK cell cytolysis. Ly-49D is an activating receptor while Ly-49A is a inhibitory receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
Ly-49A/D Antibody (12-5783-81) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse NK1.1 APC (Product # 17-5941-82) and 0.06 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse Ly-49A/D PE (right). Total viable cells were used for analysis. |
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参考文献: |
1.Journal of immunology (Baltimore, Md. : 1950)Viral MHC class I-like molecule allows evasion of NK cell effector responses in vivo."12-5783 was used in Flow cytometry/Cell sorting to show that recognition of the viral protein m157 by the inhibitory Ly49C receptor allows escape from natural killer cell control of viral replication."2.The Journal of experimental medicineDistinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains."12-5783 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection." |
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