Ly-49G Monoclonal Antibody (AT-8 (AT8)), PE, eBioscience
货号:12-5885-82
规格:100 µg
市场价格:1855
产品类型:流式抗体
品牌:eBioscience
抗原:Ly-49G
物种:小鼠
宿主:小鼠
抗体亚型:IgG2a, kappa
克隆号:AT-8 (AT8)
荧光染料:PE
类型: | 流式抗体 | 同型对照: | Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience |
浓度: | 0.2 mg/mL | 用法: | 1 μg/test(Flow) |
产品详细信息Description:
The AT8 monoclonal antibody reacts with the mouse Ly-49G receptor from 129, FVB, BALB/c, C3H, CBA, SJL and A/J, but not C57Bl/6 mice. Ly49G, a member of the Ly49 family is expressed by NK cell subset.Applications Reported: The AT-8 (AT8) antibody has been reported for use in flow cytometric analysis.Applications Tested: The AT-8 (AT8) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. Atest is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
数据 |
Ly-49G Antibody (12-5885-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD49b (Integrin alpha 2) APC (Product # 17-5971-82) and 0.25 µg of Mouse IgG2a kappa Isotype Control PE (Product # 12-4724-81) (left) or 0.5 µg of Anti-Mouse Ly-49G PE (right). Total viable cells were used for analysis. |
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参考文献: |
1.The Journal of experimental medicineDistinct MHC class I-dependent NK cell-activating receptors control cytomegalovirus infection in different mouse strains."12-5885 was used in Flow cytometry/Cell sorting to study how multiple activating Ly49 receptors may participate in H-2-dependent recognition of MCMV infection, providing a common mechanism of NK cell-mediated resistance against viral infection." |
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