CD314 (NKG2D) Monoclonal Antibody (A10), Biotin Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Biotin-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

CD314 (NKG2D) Monoclonal Antibody (A10), Biotin Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Biotin

2024-10-28

CD314 (NKG2D) Monoclonal Antibody (A10), Biotin

货号：13-5872-82,13-5872-85

规格：100μg,500μg

市场价格：2055,2892

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：Biotin

抗体类型	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin
浓度：	0.5 mg/mL	用法：	0.25 μg/test(Flow)
产品详细信息Description: The A10 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1 alpha, beta, gamma, delta, epsilon and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1(+) thymocytes, in vitro activated LAK cells, and a subset ofsplenic NKT cells. A10 and C7 antibodies detect NK cells from all inbred strains of mice tested so far. In redirected lysis assays, A10 is reported to stimulate NK cells to kill Daudi targets. In addition, immobilized A10 is reported to induce production of GM-CSF by NK clones and LAK cells through a CD16 (FC gamma RIII) concomitant engagement. A10 and another hamster anti-mouse NKG2D (clone C7) compete with each other for binding to transfected cells by flow cytometric analysis, suggesting that they may bind to similar epitopes or block each other by steric hindrance. C7 (neutralizing) and A10 (activating) also exhibit different functional properties.Expression of the NKG2D antigen on mouse peripheral NK and NKT cells can be detected by flow cytometric analysis using mAb CX5 with much brighter intensity.Applications Reported: This A10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A10 antibody has been tested by flow cytometric anlysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. NK cells can regulate specific humoral and cell-mediated immunity, and preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NK gene encodes a member of the NKG2 family, and the encoded transmembrane protein is characterized by a type II membrane orientation (extracellular Cterminus) and the presence of a C-type lectin domain. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells.
数据
CD314 (NKG2D) Antibody (13-5872-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) FITC (Product # 11-5971-82) and 0.125 µg of Armenian Hamster IgG Isotype Control Biotin (Product # 13-4888-81) (left) or 0.125 µg of Anti-Mouse CD314 (NKG2D) Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.
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参考文献：
1.OncoimmunologyIL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia developmentin vivo."13-5872 was used in Flow cytometry/Cell sorting to show that T-ALL blasts normally resistant to NK cells may be targeted by cytokine pre-activated autologous NK cells."

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CD314 (NKG2D) Monoclonal Antibody (A10), Biotin Thermo CD314 (NKG2D) Monoclonal Antibody (A10), Biotin

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