Sheep anti FIBRONECTIN 伯乐FIBRONECTIN 抗体 VPA00045

2024-10-28

Sheep anti FIBRONECTIN

货号:VPA00045

规格:100 µl

市场价格:2890

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:FIBRONECTIN

物种:人

宿主:Sheep

抗体亚型:IgG

荧光染料:Purified

产品介绍
Sheep anti Human fibronectin antibodydetects human fibronectin, a multi-domain protein involved in the connection of cells to the extracellular matrix. Fibronectin is mostly found in dimeric form, consisting of two nearly identical polypeptide chains linked by a pair of C-terminal disulfide bonds. Each fibronectin monomer has a molecular weight of 230–250 kDa. Fibronectin can bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectin is present in the body in two forms; plasma fibronectin (soluble dimeric form) is secreted by hepatocytes, whilst cellular fibronectin (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing and blood coagulation (Schwarzbauer and DiSimone, 2011) as well as pathologies such as metastasis (Van Obberghen-Schillinget al.2011).Sheep anti Human fibronectin antibody recognizes fibronectin as a single band of approximately 260 kDa in a range of human cell lysates by western blotting under reducing conditions. Cross reactivity with rodent cell line lysates is also observed.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationSheep polyclonal antibody purified by affinity chromatography.
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3).
ImmunogenHighly pure human fibronectin.
Approx. Protein ConcentrationsIgG concentration 0.5 mg/ml.
AcknowledgementsPrecisionAb is a trademark of Bio-Rad Laboratories.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store undiluted at -20°C, avoiding repeated freeze thaw cycles
应用
Application NameVerifiedMin DilutionMax Dilution
Western Blotting
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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技术参数

产品应用 WB

产品类型 PrecisionAb Polyclonal

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