Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody BIO-RAD STAR73 Phycoerythrin conjugated Goat F(ab')2 anti Rat I

2024-10-28

Phycoerythrin conjugated Goat F(ab')2 anti Rat IgG antibody

货号:STAR73

规格:0.5 ml

市场价格:4956

产品类型:流式抗体

品牌:Bio-Rad

物种:大鼠

宿主:山羊

抗体亚型:IgG

荧光染料:PE

产品介绍
Phycoerythrin conjugated Goat F(ab')2anti Rat IgG antibodyrecognises rat IgG. The reagent has been adsorbed to minimise cross-reactivity with mouse immunoglobulins and is therefore of particular value in detecting rat primary antibodies bound to mouse tissues.We recommend diluting Phycoerythrin conjugated Goat F(ab')2anti Rat IgG antibody in buffer containing 10% normal mouse serum to remove any remaining cross-reactivity.
产品详情
Target SpeciesRat
Product FormF(ab')2fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
ReconstitutionReconstitute with 0.5 ml distilled water
Antiserum PreparationAntisera to rat IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG prepared by affinity chromatography. F(ab')2 fragments were prepared by pepsin digestion
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)
ImmunogenWhole rat IgG.
Approx. Protein ConcentrationsIgG concentration 0.05mg/ml
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/51/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 106cells or 100ul human whole blood.
文献
1.Bellingan. G.et al. (2002) Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation.J. Exp. Med. 196:1515-1521.2.Broderick, C.A.et al.(2005) Local Administration of an Adeno-associated Viral Vector Expressing IL-10 Reduces Monocyte Infiltration and Subsequent Photoreceptor Damage during Experimental Autoimmune UveitisMol Ther. 12: 369-73.3.Ko, Y.C.et al.(2009) Endothelial CD200 is heterogeneously distributed, regulated and involved in immune cell–endothelium interactionsJ Anat. 214: 183-95.4.Curran, C.S.et al.(2006) Lactoferrin activates macrophages via TLR4-dependent and -independent signaling pathwaysCell Immunol. 242: 23-30.

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产品类型 多克隆抗体

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