M-CSF (Macrophage colony-stimulating factor) BIO-RAD PHP220 M-CSF (Macrophage colony-stimulating factor)

2024-10-28

M-CSF (Macrophage colony-stimulating factor)

货号:PHP220

规格:10 µg

市场价格:3162

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
M-CSF (Macrophage colony-stimulating factor)is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
PreparationPurified recombinant M-CSF expressed inE.coli
ReconstitutionReconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
ActivityThe ED50, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was ≤ 1 ng/ml corresponding to a specific activity of ≥ 1 x 106units/mg.
Preservative StabilisersNone present
Approx. Protein Concentrations0.1 mg/ml after reconstitution
Protein Molecular Weight36.8 kDa (159 amino acid homodimer)
Purity≥ 98% by SDS PAGE and HPLC
RegulatoryFor research purposes only
Guarantee3 months from date of reconstitution
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays1.0
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 wb,elisa

产品类型 细胞因子添加物

推荐用于 wb,elisa

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