Recombinant Human PAI-1 BIO-RAD PHP199 Recombinant Human PAI-1

2024-10-29

Recombinant Human PAI-1

货号:PHP199

规格:10 µg

市场价格:3162

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
PAI-1 (plasminogen activator inhibitor-1) is a serine protease inhibitor and member of the serpin family, which acts as the primary inhibitor of tissue plasminogen activator (tPA) and urokinase plasminogen activator (uPA), and hence fibrinolysis. Increased levels of PAI-1 are associated with diabetes, cardiovascular disease and an increased occurrence of thrombosis in obesity, and can be induced by obesity related factors including insulin, glucose and VLDL (very-low-density lipoprotein).
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
PreparationPurified recombinant PAI-1 expressed inE.coli.
ReconstitutionReconstitute with 100 ul distilled water.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For extended sotrage, the addition of 0.1% BSA is recommended.
Buffer SolutionSodium Acetate buffered saline
ActivityDetermined by its inhibitory effect against single chain tPA induced cleavage of a chromogenic substrate in Imidazole Buffer at 37oC. Half maximal inhibition against 1.0 μg/ml of single chain tPA was obtained at a concentration of 2.0 μg/ml.
Preservative StabilisersNone present
Protein Molecular Weight42.7 kDa (379 amino acid residues).
Purity>95% by SDS PAGE and HPLC analysis.
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FN

产品类型 重组蛋白

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