Recombinant Adiponectin BIO-RAD PHP196 Recombinant Adiponectin

2024-10-29

Recombinant Adiponectin

货号:PHP196

规格:25 µg

市场价格:3247

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Recombinant Adiponectinproducted in Hi5 cells derived from the cabbage looper mothTrichoplusia nicorresponds to amino acids 19-244 of the human adiponectin precurson and containing an N-terminal histidine tag. Adiponectin is an adipokine exclusively secreted by adipocytes, which plays a major role in the control of fat metabolism and insulin sensitivity, through the regulation of energy homeostasis and glucose and lipid metabolism. A reduced level of plasma adiponectin is associated with type 2 diabetes and obesity insulin resistance. Adiponectin is an abundant protein in normal serum corresponding to approximately 0.1% of the total protein content.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
PreparationPurified recombinant adiponectin expressed in High-5 insect cells using a baculovirus expression system
ReconstitutionReconstitute with 25 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Buffer Solution10mM TRIS, pH 8.575mM L-Arginine
ActivityDetermined by a cytotoxic assay using M1 cells. The ED50for this effect is 3.0-6.0 μg/ml.
Preservative StabilisersNone present
Protein Molecular Weight26 kDa (250 amino acid residues)
Purity>97% by SDS PAGE and HPLC
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 ELISA;FN

产品类型 重组蛋白

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