Recombinant Human TGF Beta 1 BIO-RAD PHP143B Recombinant Human TGF Beta 1

2024-10-29

Recombinant Human TGF Beta 1

货号:PHP143B

规格:10 µg

市场价格:2661

产品类型:细胞因子添加物

品牌:Bio-Rad

产品介绍
Transforming growth factor beta-1 (TGF beta 1) is a homodimeric protein that regulates the proliferation and differentiation of normal and transformed cells. TGF beta 1 is the most abundant isoform which is secreted by most cell types.
产品详情
Target SpeciesHuman
Product FormPurified recombinant protein - lyophilized
ReconstitutionReconstitute with 100ul distilled water. Further dilutions should be made in buffer containing a carrier protein such as 0.1% BSA.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. DO NOT VORTEX.
PreparationRecombinant protein prepared from CHO cells.
Preservative StabilisersNone present
ActivityThe ED50, determined by the ability of TGF beta 1 to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells is ≤0.05 ng/ml, corresponding to a specific activity of ≥2 x 107units/mg
Carrier FreeYes
Purity>98% by SDS PAGE and HPLC analysis
Protein Molecular Weight25.0 kDa (2x identical 112 amino acid polypeptide chains)
Approx. Protein Concentrations0.1 mg/ml after reconstitution
RegulatoryFor research purposes only
GuaranteeGuaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.
存储条件
Prior to reconstitution store at -20℃. Following reconstitution store at -20℃.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 FN

产品类型 重组蛋白

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