Mouse anti human CD27:StarBright Violet 610

货号：MCA755SBV610

规格：100 Tests/0.5ml

市场价格：3630

产品类型：流式抗体

品牌：Bio-Rad

抗原： CD27

物种：人

宿主：小鼠

抗体亚型：IgG2a

克隆号：LT27

荧光染料：StarBright Violet 610

类型:	单抗	同型对照：	IgG2a
浓度：		用法：	5 µL/test(Flow)
免疫原性：	Normal human blood lymphocytes.

Mouse anti human CD27, clone LT27,recognizes the human T-cell activation antigen, CD27. This antigen is expressed on activated T lymphocytes and belongs to a family of proteins associated with the nerve growth factor receptor. CD27 is expressed by thymocytes and peripheral T cells and also by EBV transformed B cell lines.Mouse anti human CD27, clone LT27 precipitates a ~55 kDa protein under reducing conditions or a ~120 kDa protein under non-reducing conditions.

数据

Figure A. PE-Cy5 conjugated Mouse anti Human CD3 (MCA463C). Figure B. PECy5.5 conjugated Mouse anti Human CD3 (MCA463C) and StarBright Violet 610 conjugated Mouse anti Human CD27 (MCA755SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

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参考文献：

1. Ravoet, A.M.et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCCin Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W.et al.Oxford University Press pp 408-411.2. Boxall, S.et al.(2004) Abnormal cell surface antigen expression in individuals with variant CD45 splicing and histiocytosis.Pediatr Res. 55 (3): 478-84.3. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.4. Becker, P.D.et al.(2010) Generation of Human Antigen-Specific Monoclonal IgM Antibodies Using Vaccinated ‘‘Human Immune System’’ MicePLoS One. 5(10). pii: e13137.5. van Zelm, M.C.et al.(2007) Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion.J Exp Med. 204: 645-55.6. Steger, B.et al.(2014) CD4(+)and CD8(+)T-cell reactions against leukemia-associated- or minor-histocompatibility-antigens in AML-patients after allogeneic SCT.Immunobiology. 219 (4): 247-60.

技术参数

产品应用 Flow

产品类型 单克隆抗体

推荐用于 Flow

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