Mouse anti Sheep CD14 monoclonal antibody, clone VPM66 BIO-RAD MCA5939GA Mouse anti Sheep CD14 monoclonal antibody, clone V-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Sheep CD14 monoclonal antibody, clone VPM66 BIO-RAD MCA5939GA Mouse anti Sheep CD14 monoclonal antibody, clone V

2024-10-29

Mouse anti Sheep CD14 monoclonal antibody, clone VPM66

货号：MCA5939GA

规格：0.1 mg

市场价格：2185

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD14

物种：绵羊

宿主：小鼠

抗体亚型：IgG1

克隆号：VPM66

荧光染料：Purified

产品介绍
Mouse anti Sheep CD14 monoclonal antibody, clone VPM66recognises ovine CD14, a GPI-anchored ~55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family. Ovine CD14 is expressed by monocytes, macrophages and peripheral blood granulocytes (Hopkinset. al.1996).CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein. The binding of LPS to CD14 results in cell activation, the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Clone VPM66 has been shown to recognise CD14 in other domestic ruminant species including Water Buffalo (Bubalus bubalis) (Vimoset. al.1996) and domestic cattle (Hopkinset. al.1996). In addition to clone VPM66, clonesVPM65andVPM67are also available.
产品详情
Target Species	Sheep
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)
Immunogen	Ovine macrophages.
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Hopkins, J. & Gupta, V.K. (1996) Identification of three myeloid-specific differentiation antigens in sheep.Vet Immunol Immunopathol. 52 (4): 329-39.2.Akesson, C.P.et al.(2008) Phenotypic characterisation of intestinal dendritic cells in sheep.Dev Comp Immunol. 32 (7): 837-49.3.Gupta, V.K.et al.(1996) Identification of the sheep homologue of the monocyte cell surface molecule--CD14.Vet Immunol Immunopathol. 51 (1-2): 89-99.4.Vilmos, P.et al.(1996) Phylogenetically conserved epitopes of leukocyte antigens.Vet Immunol Immunopathol. 52 (4): 415-26.

技术参数

产品应用 Flow；IP

产品类型 Monoclonal Antibody

推荐用于 Flow；IP

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Mouse anti Sheep CD14 monoclonal antibody, clone VPM66 BIO-RAD MCA5939GA Mouse anti Sheep CD14 monoclonal antibody, clone V

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