| 产品介绍 | | |
| Mouse anti Rat CD45 antibody, clone OX-1recognizes CD45, also known as the leucocyte common antigen (LCA). The leucocyte common antigen consists of a family of heavily glycosylated membrane glycoproteins of molecular weight 180 – 240kDa.Antibodies recognising a common epitope on all of these isoforms are termed CD45, whilst those recognising only individual isoforms are termed CD45RA, CD45RO etc. OX-1 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes.CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.This product is routinely tested in flow cytometry on rat splenocytes |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 750 - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Immunogen | Rat thymocyte membrane glycoproteins. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE-Alexa Fluor®750 488nm laser | 496 | 779 |
| Acknowledgements | This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control:RPE-Alexa Fluor® 750 | MCA1209P750 | F | 100 Tests/1ml |
| 文献 |
| 1.Dick, A.D.et al.(2001) Distribution of OX2 antigen and OX2 receptor within retina.Invest Ophthalmol Vis Sci. 42: 170-6.2.Kurozumi, K.et al.(2007) Effect of tumor microenvironment modulation on the efficacy of oncolytic virus therapy.J Natl Cancer Inst. 99: 1768-81.3.Sunderland, C.A.et al.(1979) Purification with monoclonal antibody of a predominant leukocyte-common antigen and glycoprotein from rat thymocytes.Eur J Immunol. 9 (2): 155-9.4.Woollett, G.R.et al.(1985) Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.Eur J Immunol. 15 (2): 168-73. |
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