mouse anti human F-ACTIN 伯乐 Rat anti mouse IgG HEAVY chain:Biotin MCA358G-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human F-ACTIN 伯乐 Rat anti mouse IgG HEAVY chain:Biotin MCA358G

2024-10-29

mouse anti human F-ACTIN

货号：MCA358G,MCA358GT

规格：0.5 mg,50 µg

市场价格：3766,1530

产品类型：流式抗体

品牌：Bio-Rad

抗原：Actin F Type

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：NH3

荧光染料：Biotin

产品介绍
Mouse anti Human F-Actin antibody, clone NH3recognizes human Filamentous actin (F-actin), the polymeric form of actin.. Mouse anti Human F-Actin antibody, clone NH3 binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids.In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localized nuclear staining of all cell types.
产品详情
Target Species	Human
Product Form	Purified IgM - liquid
Preparation	Purified IgM prepared from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide
Immunogen	Human monocytes and U937 cell line.
Approx. Protein Concentrations	IgM concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
ELISA	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Description	Product Code	Applications	Pack Size
Mouse IgM Negative Control	MCA692	F	100Tests
文献
1.Dransfield, I.et al. (1988) Initial characterization of an anti-actin monoclonal antibody (NH3).Biochem. Soc. Trans. 16: 163-164.2.Allen, K.M. & Haworth, S.G. (1989) Cytoskeletal features of immature pulmonary vascular smooth muscle cells: the influence of pulmonary hypertension on normal development.J Pathol. 158 (4): 311-7.3.McCarthy, A.M.et al.(2006) Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport.Am J Physiol Cell Physiol. 291: C860-8.4.Bhonagiri, P.et al.(2011) Evidence coupling increased hexosamine biosynthesis pathway activity to membrane cholesterol toxicity and cortical filamentous actin derangement contributing to cellular insulin resistance.Endocrinology. 152: 3373-84.

技术参数

产品应用 Flow;IHC(F);WB;ELISA

产品类型单克隆抗体

推荐用于 Flow;IHC(F);WB;ELISA

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mouse anti human F-ACTIN 伯乐 Rat anti mouse IgG HEAVY chain:Biotin MCA358G

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