Rat anti mouse IgG2a HEAVY chain:FITC 伯乐小鼠 IgG2a HEAVY chain MCA421F-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse IgG2a HEAVY chain:FITC 伯乐小鼠 IgG2a HEAVY chain MCA421F

2024-10-29

Rat anti mouse IgG2a HEAVY chain:FITC

货号：MCA421F

规格：0.5 mg

市场价格：5211

产品类型：荧光二抗

品牌：Bio-Rad

抗原：IgG2a

物种：小鼠

宿主：大鼠

抗体亚型：IgG1

克隆号：LO-MG2a-7

荧光染料：FITC

产品介绍
Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice).
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.1%Sodium Azide
Immunogen	Purified IgG from BALB/c mice.
Approx. Protein Concentrations	IgG concentration 1.0mg/ml
Fusion Partners	Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Jarman, E.R. & Lamb, J.R. (2004) Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling.Immunology. 112 (4): 631-42.2.Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.Prot Biol Fluids 615 - 8. Pergamon Press, Oxford and New York.3.Nagao, K.et al.(2003) Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.Am J Respir Cell Mol Biol. 29: 314-20.4.Bazin, H.et al.(1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.J Immunol Methods. 66 (2): 261-9.5.Bazin, H.et al.(1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.J Immunol Methods. 71 (1): 9-16.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Rat anti mouse IgG2a HEAVY chain:FITC 伯乐 小鼠 IgG2a HEAVY chain MCA421F

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Rat anti mouse IgG2a HEAVY chain:FITC 伯乐小鼠 IgG2a HEAVY chain MCA421F