| 产品介绍 | | |
| Mouse anti Rat CD11b, clone OX-42recognizes rat CD11b, also known asintegrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a singlevWFAdomain and multipleFG-GAPrepeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes andmicrogliain the brain (Robinsonet al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinsonet al.1986) as well as inhibit myelin binding and uptake (van der Laanet al.1996). |
| 产品详情 | | |
| Target Species | Rat |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilized |
| Preparation | Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant. |
| Buffer Solution | Phosphate buffered saline |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line. |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1%Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE-Alexa Fluor®647 488nm laser | 496 | 667 |
| Acknowledgements | This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | 1/10 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG2a Negative Control:Alexa Fluor® 647 | MCA1210A647 | F | 100 Tests/1ml |
| 文献 |
| 1.Stidworthy, M.F.et al.(2004) Notch1 and Jagged1 are expressed after CNS demyelination, but are not a major rate-determining factor during remyelination.Brain, 127: 1928-41.2.Baca Jones, C.C. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells.Virology. 388: 78-90.3.Clark, A.K.et al(2007) Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.Proc Natl Acad Sci U S A. 104: 10655-60.4.Draskovic-Pavlovic, B.et al.(1999) Differential effects of anti-rat CD11b monoclonal antibodies on granulocyte adhesiveness.Immunology. 96: 83-9. |
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