Rat anti mouse CD4:FITC 伯乐MCA4635F CD4抗体

2024-10-29

Rat anti mouse CD4:FITC

货号:MCA4635F

规格:0.1 mg

市场价格:2125

产品类型:流式抗体

品牌:Bio-Rad

物种:小鼠

宿主:大鼠

抗体亚型:IgG2b, kappa

荧光染料:FITC

产品介绍
Rat anti Mouse CD4 antibody, clone GK1.5recognizes mouse CD4, a ~55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on T helper cells, peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.Rat anti Mouse CD4 antibody, clone GK1.5 has been reported to block CD4+T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used forin vivoandin vitrocell depletion of CD4+T-cells.
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
ImmunogenMurine CD4.
Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Fusion PartnersSpleen cells from immunised Lewis rats were fused with cells of the SP2/0 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Dialynas, D.P.et al.(1983) Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule.J Immunol. 131 (5): 2445-51.2.Wilde, D.B.et al.(1983) Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines.J Immunol. 131 (5): 2178-83.3.Dialynas, D.P.et al.(1983) Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity.Immunol Rev. 74: 29-56.4.Zhou, Z.et al.(2011) Autoreactive marginal zone B cells enter the follicles and interact with CD4+ T cells in lupus-prone mice.BMC Immunol. 12: 7.

技术参数

产品应用 FLOW

产品类型 单克隆抗体

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