mouse anti PIG GRANULOCYTES:FITC 伯乐 mouse anti PIG GRANULOCYTES:FITC MCA2599F-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti PIG GRANULOCYTES:FITC 伯乐 mouse anti PIG GRANULOCYTES:FITC MCA2599F

2024-10-29

mouse anti PIG GRANULOCYTES:FITC

货号：MCA2599F

规格：0.1 mg

市场价格：3638

产品类型：流式抗体

品牌：Bio-Rad

抗原：Granulocytes

物种：pig

宿主：小鼠

抗体亚型：IgG2a

克隆号：6D10

荧光染料：FITC

产品介绍
Mouse anti Pig Granulocytes antibody, clone 6D10recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2-early myeloid precursors, 6D10+2B2-immature neutrophils, 6D10+2B2+mature neutrophils and 6D10-2B2+mature eosinophils and basophils.Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérezet al.2007).
产品详情
Target Species	Pig
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunized Balb/c mouse were fused with cells of the SP2/0 mouse myeloma cell line.
Buffer Solution	Phosphate buffered saline
Immunogen	Porcine bone marrow haematopoietic cells (BMHC).
Approx. Protein Concentrations	IgG concentration 0.1mg/ml
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Description	Product Code	Applications	Pack Size
Mouse IgG2a Negative Control:FITC	MCA929F	F	100 Tests
文献
1.Pérez, C.et al.(2007) Phenotypic and functional characterization of porcine granulocyte developmental stages using two new markers.Dev Comp Immunol. 31 (3): 296-306.2.Stone, J.P.et al.(2016) Altered Immunogenicity of Donor Lungs via Removal of Passenger Leukocytes UsingEx VivoLung Perfusion.Am J Transplant. 16 (1): 33-43.3.Ezquerra, A.et al.(2009) Porcine myelomonocytic markers and cell populations.Dev Comp Immunol. 33 (3): 284-98.4.Gardner, D.S.et al.(2016) Remote effects of acute kidney injury in a porcine model.Am J Physiol Renal Physiol. 310 (4): F259-71.4.Nguyen, D.N.et al.(2016) Delayed development of systemic immunity in preterm pigs as a model for preterm infants.Sci Rep. 6: 36816.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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mouse anti PIG GRANULOCYTES:FITC 伯乐 mouse anti PIG GRANULOCYTES:FITC MCA2599F

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