Rat anti mouse CDw199:PE 伯乐 mouse CDw199 MCA2586PE

2024-10-29

Rat anti mouse CDw199:PE

货号:MCA2586PE

规格:100 Tests

市场价格:3698

产品类型:流式抗体

品牌:Bio-Rad

抗原:CDw199

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a

克隆号:9B1

荧光染料:PE

产品介绍
Rat anti Mouse CD199 antibody, clone 9B1detects mouse chemokine receptor 9 (CCR9), also known as CD199. CCR9 is a seven transmembrane protein which is a member of the beta chemokines receptor family. CCR9 is expressed in thymus and small intestine, and mediates the recruitment and development of thymocytes in response to CCL25; the only known ligand of CCR9.CCR9 is an alternative co-receptor with CD4 for HIV infection, and has been implicated in adult T cell leukaemia. Two alternatively spliced transcript variants have been described.Clone 9B1 blocks CCL25-induced Ca2+release, attenuating inflammation in a murine model of chronic ileitis when given in early disease stages.
产品详情
Target SpeciesMouse
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Immunogen25 amino acids from the amino terminal of murine CCR9, coupled to KLH.
Approx. Protein ConcentrationsIgG concentration 0.05 mg/ml
Preservative Stabilisers0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
Fusion PartnersSpleen cells from immunised Lewis rat were fused with cells of the X63-Ag8.653 myeloma cell line.
ReconstitutionReconstitute with 1.0ml distilled water
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Rivera-nieves, J.et al.(2006) Antibody blockade of CCL25/CCR9 ameliorates early but not late chronic murine ileitis.Gastroenterology. 131 (5): 1518-29.2.Nagakubo, D.et al.(2007) Expression of CCR9 in HTLV-1+ T cells and ATL cells expressing Tax.Int J Cancer. 120 (7): 1591-7.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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