| 产品介绍 | | |
| Rat anti Mouse CD88 antibody, clone 10/92recognizes murine CD88, a 45 kDa G-protein coupled cell surface receptor, otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, a potent proinflammatory molecule and chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.Rat anti Mouse CD88 antibody, clone 10/92 does not block the binding of the C5a to murine CD88 (Souriet al.2003). |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Alexa Fluor®488 - liquid |
| Reconstitution | Reconstitute with 1 ml distilled water |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Fusion Partners | Cells from immunised Lou/c rats were fused with cells of the X63-Ag8.653 myeloma cell line. |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | RBL-2H3 transfected cells expressing murine C5aR. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Preservative Stabilisers | 0.09%Sodium Azide1%Bovine Serum Albumin |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Soruri, A.et al. (2003) Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody.Immunol Lett. 88:47-52.2.Shagdarsuren, E.et al.(2010) C5a Receptor Targeting in Neointima Formation After Arterial Injury in Atherosclerosis-Prone Mice.Circulation. 122: 1026-36.3.Manthey, H.D.et al.(2011) Complement C5a inhibition reduces atherosclerosis in ApoE-/- mice.FASEB J. 25: 2447-55.4.Cudaback, E.et al.(2011) Apolipoprotein E isoform-dependent microglia migration.FASEB J. 25: 2082-91.5.Ager, R.R.et al.(2010) Microglial C5aR (CD88) expression correlates with amyloid-beta deposition in murine models of Alzheimer's disease.J Neurochem. 113: 389-401 |
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