Rat anti mouse ER-MP58 伯乐 ER-MP58 MCA2391

2024-10-29

Rat anti mouse ER-MP58

货号: MCA2391,MCA2391T

规格:0.25 mg,25 µg

市场价格:3128,808

产品类型:流式抗体

品牌:Bio-Rad

抗原:ER-MP58

物种:小鼠

宿主:大鼠

抗体亚型:IgG2a

克隆号:ER-MP58

荧光染料:Purified

产品介绍
Rat anti Mouse ER-MP58 antibody, clone ER-MP58recognizes the murine antigen ER-MP58, which is expressed by all bone marrow-derived M-CSF- and GM-CSF-responsive myeloid blood cell precursors.The expression of ER-MP58 remains at a high level throughout the precursor/monocyte stage and is down-regulated upon maturation into mature macrophages. The ER-MP58 antigen is used to distinguish between early myeloid-committed cells, haematopoietic progenitors cells and as a marker for macrophage development in bone marrow. ER-MP58 is suitable for the identification of myeloid haemopoietic islands in various organs, and for embryonic tissues.
产品详情
Target SpeciesMouse
Product FormPurified IgG - liquid
ReconstitutionReconstitute with 1 ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion PartnersCells from immunized rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Buffer SolutionPhosphate buffered saline
ImmunogenBalb/c macrophage precursor cell hybrids
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
Preservative Stabilisers0.09%Sodium Azide
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Store at +4oC. DO NOT FREEZE.This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.2.Henkel, G.W.et al.(1999) Commitment to the monocytic lineage occurs in the absence of the transcription factor PU.1.Blood. 93 (9): 2849-58.3.Nikolic, T.et al.(2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.Int Immunol. 15 (4): 515-24.4.Geutskens, S.B.et al.(2005) Macrophages in the murine pancreas and their involvement in fetal endocrine developmentin vitro.J Leukoc Biol. 78 (4): 845-52.5.Sunderkötter, C.et al.(2004) Subpopulations of mouse blood monocytes differ in maturation stage and inflammatory response.J Immunol. 172: 4410-7.

技术参数

产品应用 IHC(F); Flow; IF;IP

产品类型 单克隆抗体

推荐用于 IHC(F); Flow; IF;IP

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