Rat anti mouse Ly-6C:Alexa Fluor 488 伯乐 mouse Ly-6C MCA2389A488-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse Ly-6C:Alexa Fluor 488 伯乐 mouse Ly-6C MCA2389A488

2024-10-29

Rat anti mouse Ly-6C:Alexa Fluor 488

货号：MCA2389A488,MCA2389A488T

规格：100 Tests/1ml,25 Tests/0.25ml

市场价格：3290,1233

产品类型：流式抗体

品牌：Bio-Rad

抗原：Ly-6C

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号：ER-MP20

荧光染料：Alexa Fluor 488

产品介绍
Rat anti Mouse Ly-6C antibody, clone ER-MP20recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chimonocytes are recruited to acute peripheral inflammation and develop into Ly-6C+exudate macrophages, whereas more mature Ly-6C-/lomonocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with cloneER-MP12in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenenet al.1990).Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designatedLY6C2. TheLY6C1gene encodes a similar protein with ~95% sequence homology to LY6C2.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor®488 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide1%Bovine Serum Albumin
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.2.Wynn, A.A.et al.(2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158 (1): 131-45.3.de Bruijn, M.F.et al.(1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.4.Revermann, M.et al.(2010) Soluble epoxide hydrolase deficiency attenuates neointima formation in the femoral cuff model of hyperlipidemic mice.Arterioscler Thromb Vasc Biol. 30: 909-14.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti mouse Ly-6C:Alexa Fluor 488 伯乐 mouse Ly-6C MCA2389A488

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