Rat anti Mouse Ly-6C:Alexa Fluor 647 伯乐Mouse Ly-6C MCA2389A647-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti Mouse Ly-6C:Alexa Fluor 647 伯乐Mouse Ly-6C MCA2389A647

2024-10-29

Rat anti Mouse Ly-6C:Alexa Fluor 647

货号：MCA2389A647,MCA2389A647T

规格：100 Tests/1ml,25 Tests/0.25ml

市场价格：3290,1216

产品类型：流式抗体

品牌：Bio-Rad

抗原：Ly-6C

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号：ER-MP20

荧光染料：Alexa Fluor 647

产品介绍
Rat anti Mouse Ly-6C antibody, clone ER-MP20recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chimonocytes are recruited to acute peripheral inflammation and develop into Ly-6C+exudate macrophages, whereas more mature Ly-6C-/lomonocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with cloneER-MP12in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenenet al.1990).Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designatedLY6C2. TheLY6C1gene encodes a similar protein with ~95% sequence homology to LY6C2.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners	Spleen cells from immunised rats were fused with cells of the Y3-Ag1.2.3 myeloma cell line.
Buffer Solution	Phosphate buffered saline
Acknowledgements	This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Approx. Protein Concentrations	IgG concentration 0.05 mg/ml
Preservative Stabilisers	0.09%Sodium Azide
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√	Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model ofYersinia pseudotuberculosisinfection.Infect Immun 78: 2329-41.2.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.3.de Bruijn, M.F.et al.(1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.4.Schatteman, G.C.et al.(2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.Am J Pathol. 177: 2002-10.

技术参数

产品应用 Flow

产品类型单克隆抗体

推荐用于 Flow

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Rat anti Mouse Ly-6C:Alexa Fluor 647 伯乐Mouse Ly-6C MCA2389A647

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