Rat anti Mouse Ly-6C:StarBright Violet 515

货号：MCA2389SBV515

规格：100 Tests/0.5ml

市场价格：3808

产品类型：流式抗体

品牌：Bio-Rad

抗原：Ly-6C

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a

克隆号：ER-MP20

荧光染料：StarBright Violet 515

类型：	单抗	同型对照：	IgG2a
浓度：		用法：	5.0 µL/test(Flow)
免疫原性：	Balb/c macrophage precursor cell hybrids.

Rat anti Mouse Ly-6C antibody, clone ER-MP20recognizes murine Ly-6C, a 131 amino acid ~14 kDa differentiation antigen, expressed on macrophage/dendritic cell precursors in mid-stage development (late CFU-M, monoblasts and immature monocytes), granulocytes, and on a wide range of endothelial cells and subpopulations of B- and T-lymphocytes.Rat anti Mouse Ly-6C antibody, clone ER-MP20 is able to distinguish multiple mouse blood monocyte subsets: immature Ly-6Chimonocytes are recruited to acute peripheral inflammation and develop into Ly-6C+exudate macrophages, whereas more mature Ly-6C-/lomonocytes are precursors for tissue macrophages and dendritic cells in steady state.Rat anti Mouse Ly-6C, clone ER-MP20 can be used in conjunction with cloneER-MP12in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (Leenenet al.1990).Rat anti Mouse Ly-6C was originally described as recognizing a protein encoded by the LY6C gene. It has subsequently become apparent that the LY6C locus demonstrates polymorphism and the LY6C gene has been re-designatedLY6C2. TheLY6C1gene encodes a similar protein with ~95% sequence homology to LY6C2.

数据：

Figure A. Alexa Fluor 488 conjugated Rat anti Mouse CD3 (MCA500A488). Figure B. Alexa Fluor 488 conjugated Rat anti Mouse CD3 (MCA500A488) and SBV515 conjugated Rat anti Mouse Ly6C (MCA2389SBV515). All experiments performed on red cell lysed mouse blood gated on live single cell lymphocytes, in the presence of 10% mouse serum. Data acquired on theZE5 Cell Analyzer.

推荐产品：

Mouse Seroblock FcR

参考文献：

1.Zhang, Y. & Bliska, J.B. (2010) YopJ-promoted cytotoxicity and systemic colonization are associated with high levels of murine interleukin-18, gamma interferon, and neutrophils in a live vaccine model ofYersinia pseudotuberculosisinfection.Infect Immun 78: 2329-41.2.Leenen, P.J.et al.(1990) Murine macrophage precursor characterization. II. Monoclonal antibodies against macrophage precursor antigens.Eur J Immunol. 20 (1): 27-34.3.de Bruijn, M.F.et al.(1998) Bone marrow cellular composition in Listeria monocytogenes infected mice detected using ER-MP12 and ER-MP20 antibodies: a flow cytometric alternative to differential counting.J Immunol Methods. 217 (1-2): 27-39.4.Schatteman, G.C.et al.(2010) Lin- Cells Mediate Tissue Repair by Regulating MCP-1/CCL-2.Am J Pathol. 177: 2002-10.5.Baumeister, T.et al.(2003) Interleukin-3Ralpha+ myeloid dendritic cells and mast cells develop simultaneously from different bone marrow precursors in cultures with interleukin-3.J Invest Dermatol. 121: 280-8.6.Devey, L.et al.(2009) Tissue-resident macrophages protect the liver from ischemia reperfusion injury via a heme oxygenase-1-dependent mechanism.Mol Ther. 17: 65-72.7.Nikolic, T.et al.(2003) Developmental stages of myeloid dendritic cells in mouse bone marrow.Int Immunol. 15: 515-24.8.Wynn, A.A.et al.(2001) Role of granulocyte/macrophage colony-stimulating factor in zymocel-induced hepatic granuloma formation.Am J Pathol. 158 (1): 131-45.9.Lesokhin, A.M.et al.(2012) Monocytic CCR2+ Myeloid-Derived Suppressor Cells Promote Immune Escape by Limiting Activated CD8 T-cell Infiltration into the Tumor Microenvironment.Cancer Res. 72: 876-86.10.Chan, J.et al.(1998) Macrophage lineage cells in inflammation: characterization by colony-stimulating factor-1 (CSF-1) receptor (c-Fms), ER-MP58, and ER-MP20 (Ly-6C) expression.Blood. 92: 1423-31.

技术参数

产品应用 Flow

© bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://gibcohyclone.cn/thread-52845.htm