Mouse anti Human Cytochrome P450 aromatase antibody, clone H4 BIO-RAD MCA2077S Mouse anti Human Cytochrome P450 aromatase

2024-10-29

Mouse anti Human Cytochrome P450 aromatase antibody, clone H4

货号:MCA2077S

规格:1 ml

市场价格:6528

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:Cytochrome P450 aromatase

物种:人

宿主:小鼠

抗体亚型:IgG2a

克隆号:H4

荧光染料:其它

产品介绍
Mouse anti Human Cytochrome P450 aromatase antibody, clone H4recognizes a conserved epitope within cytochrome P450 aromatase (P450 arom). P450 arom plays an important role in estrogen biosynthesis and is highly conserved amongst mammals.P450 arom is highly expressed in placental tissue. For tissues where there may be low expression of P450 arom, the use of microsomal extracts may improve the staining for Western blots using Mouse anti Human Cytochrome P450 aromatase antibody, clone H4 (Turneret al.2002).
产品详情
Target SpeciesHuman
Product FormConcentrated Tissue Culture Supernatant - liquid
ImmunogenSynthetic peptide corresponding to amino acids 376 - 390 of human aromatase.
Buffer SolutionPhosphate buffered saline
Fusion PartnersSpleen cells from immunised Balb/c were fused with with cells of the mouse SP20 myeloma cell line.
Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
文献
1.Wang, H.et al.(2009) The alternative noncoding exons 1 of aromatase (Cyp19) gene modulate gene expression in a posttranscriptional manner.Endocrinology. 150 (7): 3301-7.2.Inaoka, Y.et al.(2008) Regulation of P450 oxidoreductase by gonadotropins in rat ovary and its effect on estrogen production.Reprod Biol Endocrinol. 6: 62.3.Rajhans, R.et al.(2008) Modulation of in situ estrogen synthesis by proline-, glutamic acid-, and leucine-rich protein-1: potential estrogen receptor autocrine signaling loop in breast cancer cells.Mol Endocrinol. 22: 649-64.

技术参数

产品应用 IP;WB

产品类型 Monoclonal Antibody

推荐用于 IP;WB

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