Mouse anti Human CD49b antibody, clone 16B4 BIO-RAD MCA2025PE Mouse anti Human CD49b antibody, clone 16B4-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Mouse anti Human CD49b antibody, clone 16B4 BIO-RAD MCA2025PE Mouse anti Human CD49b antibody, clone 16B4

2024-10-29

Mouse anti Human CD49b antibody, clone 16B4

货号：MCA2025PE

规格：100 Tests

市场价格：3188

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD49b

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号：16B4

荧光染料：PE

产品介绍
Mouse anti Human CD49b antibody, clone 16B4recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multipleFG-GAPrepeats and a singleVWFAdomain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.
产品详情
Target Species	Human
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilizedPurified human beta 1 preparation from HT1080 fibrosarcoma cell extract
Reconstitution	Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen	Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract
Buffer Solution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Fusion Partners	Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃. Following reconstitution store at +4℃.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Fitter, S.et al.(1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.Biochem J. 338 ( Pt 1): 61-70.2.Sincock, P.M.et al.(1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.J Cell Sci. 112 ( Pt 6): 833-44.3.Eaton, C.L.et al.(2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.Prostate. 70 (8): 875-82.4.Gassmann, P.et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.Neoplasia. 11: 651-61.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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Mouse anti Human CD49b antibody, clone 16B4 BIO-RAD MCA2025PE Mouse anti Human CD49b antibody, clone 16B4

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