Mouse anti Human CD239 antibody, clone BRIC221 BIO-RAD MCA1982T Mouse anti Human CD239 antibody, clone BRIC221

2024-10-29

Mouse anti Human CD239 antibody, clone BRIC221

货号:MCA1982T,MCA1982

规格:25 µg,0.2 mg

市场价格:893,3315

产品类型:流式抗体

品牌:Bio-Rad

物种:人

宿主:小鼠

抗体亚型:IgG2b

克隆号:BRIC221

荧光染料:Purified

产品介绍
Mouse anti Human CD239 antibody, clone BRIC221recognizes human CD239, also known as Lutheran antigen or basal cell aghesion molecule.CD239 is a 597 amino acid, ~85 kDa single pass type I membrane glycoprotein. Clone BRIC221 recognizes a monomorphic determinant expresses on both the 85 and 78 kDa Lutheran (Lu) glycoforms (El Nemeret al.1998). BRIC 221 recognizes an epitope in the fourth extracellular domain of Lu glycoprotein (Parsonset al.1997). Lutheran glycoprotein is a member of the immunoglobulin superfamily and was designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
产品详情
Target SpeciesHuman
Product FormPurified IgG - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionTRIS buffered glycine
Preservative Stabilisers<0.1% Sodium Azide (NaN3)
ImmunogenHuman erythrocytes.
Approx. Protein ConcentrationsIgG concentration 1.0 mg/ml
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry1/25
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Parsons, S.F.et al.(1997) Use of domain-deletion mutants to locate Lutheran blood group antigens to each of the five immunoglobulin superfamily domains of the Lutheran glycoprotein: elucidation of the molecular basis of the Lu(a)/Lu(b) and the Au(a)/Au(b) polymorphisms.Blood. 89 (11): 4219-25.2.Sakai, E.et al.(2007) Construction of recombinant hemagglutinin derived from the gingipain-encoding gene ofPorphyromonas gingivalis, identification of its target protein on erythrocytes, and inhibition of hemagglutination by an interdomain regional peptide.J Bacteriol. 189: 3977-86.3.Bruce, L.J.et al.(2003) A band 3-based macrocomplex of integral and peripheral proteins in the RBC membrane.Blood. 101: 4180-8.

技术参数

产品应用 Elisa;WB;IF

产品类型 Monoclonal Antibody

推荐用于 Elisa;WB;IF

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