Rat anti mouse CD68:Endotoxin Low 伯乐 MCA1957EL CD68抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD68:Endotoxin Low 伯乐 MCA1957EL CD68抗体

2024-10-29

Rat anti mouse CD68:Endotoxin Low

货号：MCA1957EL

规格：0.5 mg

市场价格：6647

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD68

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：FA-11

荧光染料：其它

产品介绍
Rat anti Mouse CD68 antibody, clone FA-11recognizes mouse macrosialin, a heavily glycosylated transmembrane protein and murine homolog of human CD68, classified as a unique scavenger receptor (ScR) family member, due to the presence of a lysosome associated membrane protein (LAMP)-like domain.CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used to demonstrate the presence/localization of macrophages.Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical assays, using both frozen and paraffin-embedded tissues (Masakiet al.2003) and (Deveyet al.2009).Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
产品详情
Target Species	Mouse
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	None present.
Carrier Free	Yes
Immunogen	Purified Concanavalin A acceptor glycoprotein from P815 cell line.
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Endotoxin Level	<0.01EU/ug
Regulatory	For research purposes only
Guarantee	12 monthsfrom dateof despatch
存储条件
Store at -20°C only.This product should be stored undiluted.Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry1	√	1/50	1/100
Immunofluorescence	√
Immunohistology - Frozen	√
Immunohistology - Paraffin 2	√
Immunoprecipitation	√
Western Blotting3	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product CodeBUF09) for this purpose.2This product may require antigen retrieval using heat treatment prior to staining of paraffin sections. Either sodium citrate buffer or Tris/EDTA buffer may be used for this purpose (Martin-Mansoet al. 2008). Staining has also been achieved without antigen retrieval (Luet al. 2010).3Non-reducing conditions recommended.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10μl of the suggested working dilution to label 1x106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:Low Endotoxin	MCA1212EL	Flow	0.5mg
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Mouse Seroblock FcR	BUF041A	Flow	0.1 mg
Mouse Seroblock FcR	BUF041B	Flow	0.5 mg
Leucoperm	BUF09	Flow	50 Tests
文献
1. Bardin, M.et al.(2022) The resolvin D2 - GPR18 axis is expressed in human coronary atherosclerosis and transduces atheroprotection in apolipoprotein E deficient mice.Biochem Pharmacol. : 115075.2. Miller-Rhodes, P.et al.(2022) URMC-099 prophylaxis prevents hippocampal vascular vulnerability and synaptic damage in an orthopedic model of delirium superimposed on dementia.FASEB J. 36 (6): e22343.3. Kovács, A.D.et al.(2022) Acidified drinking water attenuates motor deficits and brain pathology in a mouse model of a childhood neurodegenerative disorder.Sci Rep. 12 (1): 9025.4. Agrawal, S.et al.(2022) Impact of IL-21-associated peripheral and brain crosstalk on the Alzheimer's disease neuropathology.Cell Mol Life Sci. 79 (6): 331.5. Piec, P.A.et al.(2022) Muramyl Dipeptide Administration Delays Alzheimer's Disease Physiopathology via NOD2 Receptors.Cells. 11 (14): 2241.6. Yamasaki, T.et al.(2022) Inhibition of microRNA-33b specifically ameliorates abdominal aortic aneurysm formation via suppression of inflammatory pathways.Sci Rep. 12 (1): 11984.7. Burger, F.et al.(2022) The E3 Ubiquitin Ligase Peli1 Deficiency Promotes Atherosclerosis Progression.Cells. 11 (13): 2014.8. Lam, S.et al.(2022) Pathological changes induced by Alzheimer's brain inoculation in amyloid-beta plaque-bearing mice.Acta Neuropathol Commun. 10 (1): 112.9. Uchida, H.A.et al.(2022) Edaravone Attenuated Angiotensin II-Induced Atherosclerosis and Abdominal Aortic Aneurysms in Apolipoprotein E-Deficient Mice.Biomolecules. 12(8):1117.

技术参数

产品应用 IHC(F); Flow *； IF ;IP; IHC(P) * ;WB *

产品类型 Monoclonal Antibody

推荐用于 IHC(F); Flow *； IF ;IP; IHC(P) * ;WB *

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Rat anti mouse CD68:Endotoxin Low 伯乐 MCA1957EL CD68抗体

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