mouse anti Rat IgG1 HEAVY chain:FITC 伯乐MCA194F IgG1抗体

2024-10-29

mouse anti Rat IgG1 HEAVY chain:FITC

货号:MCA194F

规格:0.5 mg

市场价格:4905

产品类型:流式抗体

品牌:Bio-Rad

抗原:IgG1

物种:大鼠

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:MARG1-2

荧光染料:FITC

产品介绍
Mouse anti rat IgG1, clone MARG1-2recognizes the heavy chain of the rat immunoglobulin G1 subclass. No cross-reactivity has been found with other rat immunoglobulin classes or subclasses.
产品详情

Target Species

Rat

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.1% Sodium Azide

50% Glycerol

Immunogen

Rat IR27 IgG1 myeloma protein

Approx. Protein ConcentrationsIgG concentration 1 mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom dateof despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution

Flow Cytometry

5ug/ml
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 50ul of the suggested working dilution to label 106cells in 100ul.
同型
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:FITCMCA1209F

Flow

0.1 mg
文献
1. Pelegrí, C.et al.(2001) Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells.Clin Exp Immunol. 125 (3): 470-7.2. Sato, K.et al.(2001) Carbon monoxide generated by heme oxygenase-1 suppresses the rejection of mouse-to-rat cardiac transplants.J Immunol. 166 (6): 4185-94.3. Bézie, S.et al.(2015) Fibrinogen-Like Protein 2/Fibroleukin Induces Long-Term Allograft Survival in a Rat Model through Regulatory B Cells.PLoS One. 10 (3): e0119686.4. Bézie, S.et al.(2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.J Immunol. 195 (12): 5805-15.5. Ueta, H.et al.(2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleenInternational Immunology. 30 (2): 53-67.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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