Mouse anti Human CD166 antibody, clone 3A6 BIO-RAD MCA1926FT Mouse anti Human CD166 antibody, clone 3A6

2024-10-29

Mouse anti Human CD166 antibody, clone 3A6

货号:MCA1926FT,MCA1926F

规格:25 µg,0.1 mg

市场价格:893,3162

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD166

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号:3A6

荧光染料:FITC

产品介绍
Mouse anti Human CD166 antibody, clone 3A6recognizes the 100 kDa adhesion molecule CD166, also known as ALCAM. CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for ALCAM (Skonieret al.1996).Mouse anti Human CD166 antibody, clone 3A6 is reported to cross-react with CD166 on ovine tissues and provides a useful tool for the identification and characterization of ovine mesenchymal stem cells in conjunction withCD44which is expressed by this cell lineage and the hematopoietic cell markerCD45which is not expressed on mesenchymal stem cells (Sanjurjo-Rodríguezet al.2017).
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Species Cross-ReactivityTarget SpeciesCross Reactivity
Sheep
Preparation
  • MCA1926F: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
  • MCA1926FT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.05mg/ml
Fusion PartnersSpleen cells from immunised mice were fused with cells of the P3X63 Ag8 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Yeh, S.P.et al.(2005) Mesenchymal stem cells can be easily isolated from bone marrow of patients with various haematological malignancies but the surface antigens expression may be changed after prolongedex vivoculture.Leukemia. 19: 1505-7.2.Patel, D. D.et al. (1997) CD166 Workshop: Tissue distribution and functional analysis of antibodies reactive for CD166, a ligand for CD6.In Leukocyte Typing IV. Kishimoto, T.et al. eds Garland publishing Inc. New York p. 461-4.3.Tondreau, T.et al.(2008) Gene expression pattern of functional neuronal cells derived from human bone marrow mesenchymal stromal cells.BMC Genomics. 9:166.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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