mouse anti human CD161:FITC 伯乐MCA1855F CD161抗体

2024-10-29

mouse anti human CD161:FITC

货号:MCA1855F

规格:0.1 mg

市场价格:2933

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD161

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:B199.2

荧光染料:FITC

产品介绍
Mouse anti Human CD161 antibody, clone B199.2recognizes the human Killer cell lectin-like receptor subfamily B member 1, also known as CD161, C-type lectin domain family 5 member B, HNKR-P1a, NKR-P1A or Natural killer cell surface protein P1A. CD161 is a 225 amino acid ~25 kDa predicted molecular mass, single pass type II transmembrane glycoprotein with a singleC-type lectindomain. CD161 is expressed by almost all NK cells and a subset of CD3+ve T cells (Lanier 1994).CD161, a member of the C-lectin is expressed as a disulphide bond-linked homodimeric cell surface protein, comprising two chains of ~40-44 kDa (Lanieret al.1994). CD161 acts as a receptor for another c-type lectin, LLT1 with roles in the regulation of NK cell and T cell function (Aldemiret al.2005).Mouse anti Human CD161 antibody, clone B199.2 cross-competes with and recognizes a similar epitope to the DX1 monoclonal antibody (Lanieret al.1994).
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to FITC- liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin

ImmunogenPurified human NK cells cultured in IL-2 (Bennettet al.1996)
Approx. Protein Concentrations

IgG concentration 0.1mg/ml

Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from ligh
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNt - 1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
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DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:FITCMCA928FFlow100 Tests
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文献
1. Bennett, I.M.et al.(1996) Definition of a natural killer NKR-P1A+/CD56-/CD16- functionally immature human NK cell subset that differentiatesin vitroin the presence of interleukin 12.J Exp Med. 184 (5): 1845-56.2. Azzoni, L.et al.(1998) Differential transcriptional regulation of CD161 and a novel gene, 197/15a, by IL-2, IL-15, and IL-12 in NK and T cells.J Immunol. 161 (7): 3493-500.3. de Lalla, C.et al.(2011) Invariant NKT Cell Reconstitution in Pediatric Leukemia Patients Given HLA-Haploidentical Stem Cell Transplantation Defines Distinct CD4+ and CD4- Subset Dynamics and Correlates with Remission State.J Immunol. 186: 4490-9.4. Huarte, E.et al.(2008) PILAR is a novel modulator of human T-cell expansion.Blood. 112: 1259-68.5. Williams, P.J.et al.(2009) Altered decidual leucocyte populations in the placental bed in pre-eclampsia and foetal growth restriction: a comparison with late normal pregnancy.Reproduction. 138: 177-84.6. Higai, K.et al.(2006) Binding of sialyl Lewis X antigen to lectin-like receptors on NK cells induces cytotoxicity and tyrosine phosphorylation of a 17-kDa protein.Biochim Biophys Acta. 1760 (9): 1355-63.7. Birchall, M.A.et al.(2008) Immunologic response of the laryngeal mucosa to extraesophageal reflux.Ann Otol Rhinol Laryngol. 117: 891-5.8. Bossard, C.et al.(2012) Plasmacytoid dendritic cells and Th17 immune response contribution in gastrointestinal acute graft-versus-host disease.Leukemia. 26: 1471-4.9. Richter, J.et al.(2010) CD161 receptor participates in both impairing NK cell cytotoxicity and the response to glycans and vimentin in patients with rheumatoid arthritis.Clin Immunol. 136: 139-47.10. Abrahamsson, S.V.et al.(2013) Non-myeloablative autologous haematopoietic stem cell transplantation expands regulatory cells and depletes IL-17 producing mucosal-associated invariant T cells in multiple sclerosis.Brain. 136: 2888-903.11. Rother, S.et al.(2015) The c.503T>C Polymorphism in the Human KLRB1 Gene Alters Ligand Binding and Inhibitory Potential of CD161 Molecules.PLoS One. 10 (8): e0135682.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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