mouse anti human CD81 伯乐 MCA1847T CD81抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human CD81 伯乐 MCA1847T CD81抗体

2024-10-29

mouse anti human CD81

货号：MCA1847T ,MCA1847

规格：25 µg,0.2 mg

市场价格：893,3783

产品类型：流式抗体

品牌：Bio-Rad

抗原：CD81

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：1D6

荧光染料：Purified

产品介绍
Mouse anti Human CD81 antibody, clone 1D6recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation.Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects.
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Chimpanzee	√
Sheep	√
Goat	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide
Carrier Free	Yes
Immunogen	OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
Approx. Protein Concentrations	IgG concentration 1.0 mg/ml
Fusion Partners	Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line.
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry1	√	1/5	1/10
Immunofluorescence	√
Immunohistology - Paraffin	√
Immunoprecipitation	√
Western Blotting	√
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Use 10ul of the suggested working dilution to label 106 cells in 100ul.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control	MCA928	Flow	100 Tests/1ml
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文献
1. Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.J Immunol. 151 (8): 4090-7.2. Levy, S.et al.(1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.Annu Rev Immunol. 16: 89-109.3. Griebel, P.J.et al.(2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.Vet Immunol Immunopathol. 119: 115-22.4. Welton, J.L.et al(2010) Proteomics analysis of bladder cancer exosomes.Mol Cell Proteomics. 9: 1324-38.5. Davis, W.C.et al.(2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.Vet Immunol Immunopathol. 119: 123-30.6. Flint, M.et al.(1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.J Virol. 73:6235-44.7. Parthasarathy, V.et al.(2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.Immunology. 127: 237-48.8. Rohlena, J.et al.(2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.Cardiovasc Res. 81: 187-96.9. Ventress, J.K.et al.(2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.PLoS One. 11 (7): e0160387.10. Mleczko, J.et al.(2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print].

技术参数

产品应用 Flow; IF; IP; IHC(P); WB

产品类型 Monoclonal Antibody

推荐用于 Flow; IF; IP; IHC(P); WB

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mouse anti human CD81 伯乐 MCA1847T CD81抗体

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