| 产品介绍 | | |
| Hamster anti Mouse CD81 antibody, clone Eat2recognizes mouse and rat CD81, also known as TAPA-1 or Target of the antiproliferative antibody 1. CD81 is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. Hamster anti Mouse CD81 antibody, clone Eat2 induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maeckeret al.2000). Eat 2 requires the presence of both extracellular loops of TAPA-1 for binding.Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubensteinet al.2006). |
| 产品详情 | | |
Target Species | Mouse |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Rat | √ |
| N.B. Antibody reactivity and working conditions may vary between species. |
| Product Form | Purified IgG conjugated to Alexa Fluor® 647 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1% Bovine Serum Albumin |
| Immunogen | 38C13, murine B cell line. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Fusion Partners | Spleen cells from immunised Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®647 | 650 | 665 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | Neat |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewedpublications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR
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| 文献 |
| 1. Conde-Vancells, J.et al.(2008) Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes.J Proteome Res. 7: 5157-66.2. Takeda, Y.et al.(2008) Double deficiency of tetraspanins CD9 and CD81 alters cell motility and protease production of macrophages and causes chronic obstructive pulmonary disease-like phenotype in mice.J Biol Chem. 283: 26089-97.3. Suzuki, M.et al.(2009) Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.J Immunol. 182: 6485-93.4. Ha, C.T.et al.(2005) Binding of pregnancy-specific glycoprotein 17 to CD9 on macrophages induces secretion of IL-10, IL-6, PGE2, and TGF-beta1.J Leukoc Biol. 77: 948-57.5. Pan, Q.et al.(2011) Hepatic cell-to-cell transmission of small silencing RNA can extend the therapeutic reach of RNA interference (RNAi).Gut. 61: 1330-9.6. Jin, Y.et al.(2013) Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.PLoS One. 8: e73706.7. Royo, F.et al.(2013) Transcriptome of extracellular vesicles released by hepatocytes.PLoS One. 8: e68693.8. Owens, D.M. and Watt, F.M. (2001) Influence of beta1 integrins on epidermal squamous cell carcinoma formation in a transgenic mouse model: alpha3beta1, but not alpha2beta1, suppresses malignant conversion.Cancer Res. 61: 5248-54.9. Jin, Y.et al.(2018) Double deletion of tetraspanins CD9 and CD81 in mice leads to a syndrome resembling accelerated aging.Sci Rep. 8 (1): 5145.10. Sosa, L.J.et al.(2013) Amyloid precursor protein is an autonomous growth cone adhesion molecule engaged in contact guidance.PLoS One. 8 (5): e64521. |
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