| 产品介绍 | | |
| Rat anti Human HER2/neu antibody, clone ICR55recognizes epitope E of human HER2/neu, otherwise known as CD340 and c-ErbB-2, a proto-oncogene expressed at the cell surface of a range of tumor cells, often associated with poor prognosis, and an increased risk of metastasis.HER2/neu is used in conjunction with antibodies recognizing both Estrogen and Progesterone receptors, for the classification of breast cancer tumors, and to determine patient prognosis and course of treatment.Excessive HER2/neu is present in around 20% to 25% of invasive breast cancers, referred to as HER2-positive cancer, and can help determine whether drugs such as trastuzumab (Herceptin) or lapatinib (Tykerb), might be beneficial. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG conjugated to Alexa Fluor® 488 - liquid |
| Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
| Reconstitution | Reconstitute with 1.0 ml distilled water |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
| Immunogen | Extracellular domain of human EGF-receptor from head and neck carcinoma. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| Alexa Fluor®488 | 495 | 519 |
| Acknowledgements | This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| Description | Product Code | Applications | Pack Size |
| Rat IgG2a Negative Control:Alexa Fluor® 488 | MCA6005A488 | F | 0.1mg |
| 文献 |
| 1.Styles, J.M.et al.(1990) Rat monoclonal antibodies to the external domain of the product of the C-erbB-2 proto-oncogene.Int J Cancer. 45 (2): 320-4.2.Dean, C.J.et al.(1993) Rat MAbs to the product of the c-erbB-2 proto-oncogene for diagnosis and therapy in breast cancer.Cell Biophys. 22 (1-3): 111-27.3.Shah, A.J.et al.(2014) Non-invasive molecular profiling of cancer using photoacoustic imaging of functionalized gold nanorodsProc. of SPIE Vol. 8943, 89435G |
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