Mouse anti Pig CD31:Biotin bio-rad MCA1746B Mouse anti Pig CD31:Biotin

2024-10-29

Mouse anti Pig CD31:Biotin

货号:MCA1746B

规格:0.1 mg

市场价格:3783

产品类型:流式抗体

品牌:Bio-Rad

抗原:CD31

物种:pig

宿主:小鼠

抗体亚型:IgG1

克隆号:LCI-4

荧光染料:Biotin

产品介绍
Mouse anti Pig CD31, clone LCI-4recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwettet al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5thextracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasuet al.1999).Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasuet al.1999).
产品详情
Target SpeciesPig
Species Cross-ReactivityTarget SpeciesCross Reactivity
Human
Mouse×
N.B. Antibody reactivity and working conditions may vary between species.
Product FormPurified IgG conjugated to Biotin - liquid
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin
Approx. Protein ConcentrationsIgG concentration 0.5 mg/ml
ImmunogenPorcine CD31/human IgGFc fusion protein.
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/200
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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文献
1.Nasu, K.et al.(1999) Alpha-galactosyl-mediated activation of porcine endothelial cells: studies on CD31 and VE-cadherin in adhesion and signaling.Transplantation. 68: 861-7.2.Evans, P.C.et al. (2001) Signaling through CD31 protects endothelial cells from apoptosis.Transplantation. 71 (3): 343-4.3.Gesslein, B.et al.(2010) Mitogen-activated protein kinases in the porcine retinal arteries and neuroretina following retinal ischemia-reperfusion.Mol Vis. 16: 392-407.4.Gyöngyösi, M.et al. (2010) Differential effect of ischaemic preconditioning on mobilisation and recruitment of haematopoietic and mesenchymal stem cells in porcine myocardial ischaemia-reperfusion.Thromb Haemost. 104 (2): 376-84.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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