Mouse anti Human CD55:RPE BIO-RAD MCA1614PE Mouse anti Human CD55:RPE

2024-10-29

Mouse anti Human CD55:RPE

货号:MCA1614PE

规格:100 Tests

市场价格:3936

产品类型:免疫组化一抗

品牌:Bio-Rad

抗原:CD55

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

荧光染料:PE

产品介绍
Mouse anti Human CD55 antibody, clone 67recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types.CD55 is the complement regulatory protein, decay accelerating factor (DAF) (Lublin and Atkinson 1989). Human CD55 is a ~70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the
产品详情
Target SpeciesHuman
Product FormPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
ReconstitutionReconstitute with 1.0 ml distilled water
PreparationPurified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer SolutionPhosphate buffered saline
ImmunogenK562 cells
Preservative Stabilisers0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
RPE 488nm laser496578
RegulatoryFor research purposes only
Guarantee12 months from date of despatch
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at +4℃.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light
应用
Application NameVerifiedMin DilutionMax Dilution
Flow CytometryNeat1/2
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
同型对照
DescriptionProduct CodeApplicationsPack Size
Mouse IgG1 Negative Control:RPEMCA928PEF100 Tests
文献
1.Hadam, M.R. (1989) In Leucocyte Typing IV: White Cell Differentiation Antigens.Edited by Knapp, W.et al. Oxford University Press pp 694-697.2.Holmes, C.H.et al.(1990) Preferential expression of the complement regulatory protein decay accelerating factor at the fetomaternal interface during human pregnancy.J Immunol. 144 (8): 3099-105.3.O'Brien, D.P.et al.(2009) Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.J Biol Chem. 283: 23922-30.4.Yang, P.et al.(2009) Expression and modulation of RPE cell membrane complement regulatory proteins.Invest Ophthalmol Vis Sci. 50: 3473-81.

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