mouse anti human IgM:FITC 伯乐 MCA1662F CD45抗体

2024-10-29

mouse anti human IgM:FITC

货号:MCA1662F

规格:0.1 mg

市场价格:2865

产品类型:流式抗体

品牌:Bio-Rad

抗原: IgM

物种:人

宿主:小鼠

抗体亚型:IgG1

克隆号: M15/8

荧光染料:FITC

产品介绍
Mouse anti Human IgM monoclonal antibody, clone M15/8recognizes human IgM, binding to the μ heavy chain. The antibody does not cross-react with other immunoglobulin classes.
产品详情

Target Species

Human

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

BufferSolution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide

1% Bovine Serum Albumin

Approx. Protein ConcentrationsIgG concentration 0.1 mg/ml
Fusion PartnersSpleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/EmFluorophoreExcitation Max (nm)Emission Max (nm)
FITC490525

Regulatory

For research purposes only

Guarantee

12 monthsfrom date of despatch

存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application NameVerifiedMin DilutionMax Dilution
Flow Cytometry

Neat

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  • Flow CytometryUse 10ul of the suggested working dilution to label 106cells in 100ul
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DescriptionProduct CodeApplicationsPack Size
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文献

1. Cragg, M.S.et al.(1999) Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells.Br J Cancer. 79 (5-6): 850-7.2. Irie, E.et al. (2010) Severe hypogammaglobulinemia persisting for 6 years after treatment with rituximab combined chemotherapy due to arrest of B lymphocyte differentiation together with alteration of T lymphocyte homeostasis.Int J Hematol. 91: 501-8.3. Creasey, A.M.et al.(2003) Nonspecific immunoglobulin M binding and chondroitin sulfate A binding are linked phenotypes of Plasmodium falciparum isolates implicated in malaria during pregnancy.Infect Immun. 71: 4767-71.4. Ghumra, A.et al.(2012) Induction of strain-transcending antibodies against Group A PfEMP1 surface antigens from virulent malaria parasites.PLoS Pathog. 8 (4): e1002665.5. Semblat, J.P.et al.(2015) Identification of the minimal binding region of a Plasmodium falciparum IgM binding PfEMP1 domain.Mol Biochem Parasitol. 201 (1): 76-82.

技术参数

产品应用 Flow

产品类型 Monoclonal Antibody

推荐用于 Flow

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