mouse anti human Interferon GAMMA:FITC 伯乐 MCA1554F IFN-γ抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

mouse anti human Interferon GAMMA:FITC 伯乐 MCA1554F IFN-γ抗体

2024-10-29

mouse anti human Interferon GAMMA:FITC

货号：MCA1554F

规格：0.1 mg

市场价格：3162

产品类型：流式抗体

品牌：Bio-Rad

抗原：IFN-γ

物种：人

宿主：小鼠

抗体亚型：IgG1

克隆号： B27

荧光染料：FITC

产品介绍
Mouse anti Human Interferon gamma antibody, clone B27recognizes human interferon gamma (IFN gamma). IFN gamma is a regulatory cytokine, which is produced by activated natural killer cells and a population of T-lymphocytes.Mouse anti Human Interferon gamma antibody, clone B27 is reported to neutralize the antiviral activity of human IFN gamma (Favreet al.1989).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Rhesus Monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09%Sodium Azide 1%Bovine Serum Albumin
Approx. Protein Concentrations	IgG concentration 0.1 mg/ml
Immunogen	Recombinant human IFN gamma.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry1	√	Neat	1/10
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 1x10⁶cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Mouse IgG1 Negative Control:FITC	MCA928F	Flow	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	Flow	50 Tests
Human Seroblock	BUF070B	Flow	200 Tests
数据
Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A)for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (MCA1553PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
文献
1. Favre, C.et al.(1989) Epitope mapping of recombinant human gamma interferon using monoclonal antibodies.Mol Immunol. 26 (1): 17-25.2. Abrams, J.S.et al.(1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.Immunol Rev. 127: 5-24.3. Paliard, X.et al.(1988) Simultaneous production of IL-2, IL-4, and IFN-gamma by activated human CD4+ and CD8+ T cell clones.J Immunol. 141 (3): 849-55.4. Gomes, J.A.et al.(2003) Evidence that development of severe cardiomyopathy in human Chagas' disease is due to a Th1-specific immune response.Infect Immun. 71 (3): 1185-93.5. Lai, X.et al.(2003) Immune biology of macaque lymphocyte populations during mycobacterial infection.Clin Exp Immunol. 133: 182-92.6. Wakkach, A.et al.(2001) Differentiation of regulatory T cells 1 is induced by CD2 costimulation.J Immunol. 167: 3107-13.

技术参数

产品应用 Flow Cytometry

产品类型 Monoclonal Antibody

推荐用于 Flow Cytometry

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mouse anti human Interferon GAMMA:FITC 伯乐 MCA1554F IFN-γ抗体

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