Rat anti mouse CD134:PE 伯乐 MCA1420PE CD134抗体-试剂- bio-rad伯乐一级代理 |伯乐Aminex |伯乐电泳槽1658001| 伯乐ddPCR bio-rad授权代理商、biorad伯乐qpcr仪

Rat anti mouse CD134:PE 伯乐 MCA1420PE CD134抗体

2024-10-29

Rat anti mouse CD134:PE

货号：MCA1420PE

规格：100 Tests

市场价格：3188

产品类型：流式抗体

品牌：Bio-Rad

抗原： CD134

物种：小鼠

宿主：大鼠

抗体亚型：IgG1

克隆号：OX-86

荧光染料：PE

产品介绍
Rat anti Mouse CD134 antibody, clone OX-86recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
产品详情
Target Species	Mouse
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
Immunogen	CHO cells transfected with murine OX40.
Approx. Protein Concentrations	Pack Size: 0.1 mg IgG concentration 0.1 mg/ml Pack Size: 0.5 mg IgG concentration 0.5 mg/ml
Fusion Partners	Spleen cells from immunized AO rats were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. Flow Cytometry Use 10ul of the suggested working dilution to label 1 x10⁶cells in 100ul.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Staining of Concanavalin A stimulated mouse spleen cells with Rat anti Mouse CD134:RPE (MCA1420PE).
相关产品
Description	Product Code	Applications	Pack Size
Mouse Seroblock FcR	BUF041A	F	0.1 mg
Mouse Seroblock FcR	BUF041B	F	0.5 mg
文献
1. Maenz, M.et al.(2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.Mol Vis. 17: 420-9.2. Zaini, J.et al.(2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.J Clin Invest. 117: 3330-8.3. Andarini, S.et al.(2004) Adenovirus vector-mediated in vivo gene transfer of OX40 ligand to tumor cells enhances antitumor immunity of tumor-bearing hosts.Cancer Res. 64: 3281-7.4. Malmström, V.et al.(2001) CD134L expression on dendritic cells in the mesenteric lymph nodes drives colitis in T cell-restored SCID mice.J Immunol. 166: 6972-81.5. McHugh, R.S.et al.(2002) CD4(+)CD25(+) immunoregulatory T cells: gene expression analysis reveals a functional role for the glucocorticoid-induced TNF receptor.Immunity. 16: 311-23.6. Thaunat, O.et al.(2010) Immune responses elicited in tertiary lymphoid tissues display distinctive features.PLoS One. 5: e11398.7. Wythe, S.E.et al.(2012) OX40 Ligand and Programmed Cell Death 1 Ligand 2 Expression on Inflammatory Dendritic Cells Regulates CD4 T Cell Cytokine Production in the Lung during Viral DiseaseJ Immunol. 188: 1647-55.8. Reynolds, C.et al.(2014) Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.BMC Biol. 12: 32.

技术参数

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Rat anti mouse CD134:PE 伯乐 MCA1420PE CD134抗体

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