产品介绍

类型:

一抗

同型对照：

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience

浓度：

5 µL/Test

用法：

5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD86 (B7-2) Antibody (12-0869-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control PE (Product # 12-4732-81) (blue histogram) or Anti-Human CD86 (B7-2) PE (purple histogram). Cells in the monocyte gate were used for analysis.

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1. VirulenceAntibodies to P-selectin glycoprotein ligand-1 block dendritic cell-mediated enterovirus 71 transmission and prevent virus-induced cells death."12-0869 was used in Flow cytometry/Cell sorting to demonstrate that PSGL-1 present on the cell surfaces of monocyte-derived dendritic cells mediates enterovirus 71 binding and facilitates further infection."AuthorsRen XX,Li C,Xiong SD,Huang Z,Wang JH,Wang HBYear2016SpeciesHuman

2. Biology openAn in vitro model of granuloma-like cell aggregates substantiates early host immune responses against Mycobacterium massiliense infection."12-0869 was used in Flow cytometry/Cell sorting to study the interaction between host cells and Mycobacterium massiliense."AuthorsJe S,Quan H,Na Y,Cho SN,Kim BJ,Seok SHYear2016SpeciesHuman

3. Cancer researchPharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes."12-0869 was used in Flow cytometry/Cell sorting to investigate the consequences of inhibiting lysine-specific demethylase 1 in a panel of cell lines representing all acute myelogenous leukaemia subtypes."AuthorsMcGrath JP,Williamson KE,Balasubramanian S,Odate S,Arora S,Hatton C,Edwards TM,O'Brien T,Magnuson S,Stokoe D,Daniels DL,Bryant BM,Trojer PYear2016SpeciesHuman

4. PloS oneInterferon beta and vitamin D synergize to induce immunoregulatory receptors on peripheral blood monocytes of multiple sclerosis patients."12-0869 was used in Flow cytometry/Cell sorting to investigate whether the immunoregulatory receptors immunoglobulin-like transcript 3 and 4 mediate the therapeutic effect of interferon beta in multiple sclerosis."AuthorsWaschbisch A,Sanderson N,Krumbholz M,Vlad G,Theil D,Schwab S,Mäurer M,Derfuss TYear2015SpeciesHuman

5. BioMed research internationalDendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients."12-0869 was used in Flow cytometry/Cell sorting to investigate the dendritic cell profile induced by Schistosoma mansoni antigen in cutaneous leishmaniasis patients."AuthorsLopes DM,Fernandes JS,Cardoso TM,Bafica AM,Oliveira SC,Carvalho EM,Araujo MI,Cardoso LS

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