产品介绍

类型:

一抗

同型对照：

Mouse IgG2b kappa Isotype Control (eBMG2b), PE-Cyanine5, eBioscience

浓度：

5 µL/Test

用法：

5 µL (0.25 µg)/test(Flow）

产品详细信息Description: The IT2.2 monoclonal antibody reacts with human CD86, an ~80 kDa surface receptor also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed at low levels on B cells, macrophages, and dendritic cells and is upregulated on B cells through a variety of surface stimuli including the BCR complex, CD40 and some cytokine receptors. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.Applications Reported: The IT2.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IT2.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is theligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.仅用于科研。不用于诊断过程。

数据

CD86 (B7-2) Antibody (15-0869-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b Isotype Control PE-Cyanine5 (Product # 15-4732-42) (blue histogram) or Anti-Human CD86 (B7-2) PE-Cyanine5 (purple histogram).Cells in the monocyte gate were used for analysis.

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1. Frontiers in immunologyLeishmania infantum and Leishmania braziliensis: Differences and Similarities to Evade the Innate Immune System."15-0869 was used in Flow cytometry/Cell sorting to investigate the similarities and differences between the ways in which leishmania infantum and leishmania braziliensis evade the innate immune system.."AuthorsFalcão Sde A,Jaramillo TM,Ferreira LG,Bernardes DM,Santana JM,Favali CBYear2016SpeciesHuman

2. BloodOmental implantation of BOECs in hemophilia dogs results in circulating FVIII antigen and a complex immune response."15-0869 was used in Flow cytometry/Cell sorting to investigate the immune response to treatment with FVIII-expressing autologous BOECs."AuthorsOzelo MC,Vidal B,Brown C,Notley C,Hegadorn C,Webster S,Harpell L,Ahlin J,Winterborn A,Handforth J,Arruda VR,Hough C,Lillicrap DYear2014SpeciesHuman

3. Journal of leukocyte biologyEffects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors."15-0869 was used in Flow cytometry/Cell sorting to investigate how NLRs represent a new pathway for B cell activation and a potentially important host defense system against bacterial infections."AuthorsPetterson T,Jendholm J,Månsson A,Bjartell A,Riesbeck K,Cardell LOYear2011SpeciesHuman

4. Journal of immunological methodsNaïve and memory B cells in the rhesus macaque can be differentiated by surface expression of CD27 and have differential responses to CD40 ligation."15-0869 was used in Flow cytometry/Cell sorting to provide evidence that Rhesus macaque naive and memory B cells can be differentiated using surface expression of CD27."AuthorsKuhrt D,Faith S,Hattemer A,Leone A,Sodora D,Picker L,Borghesi L,Cole KSYear2011SpeciesRhesus monkey

5. Mediators of inflammationLinking innate and adaptive immunity: human Vgamma9Vdelta2 T cells enhance CD40 expression and HMGB-1 secretion."15-0869 was used in Flow cytometry/Cell sorting to show that gammadelta T cells induce an early upregulation of CD40 on monocytes and the local release of High Mobility Group Box-1."AuthorsKalyan S,Chow AW

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