产品介绍 抗体类型 一抗同型对照：Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor 710浓度：5 µL/Test用法：5 μL (0.06 μg)/test (Flow)产品详细信息Description: The L243 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Tested: This L243 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.数据HLA-DR Antibody (46-9952-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (blue histogram) or Anti-Human HLA-DR PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体参考文献：1.Oncology lettersAccumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients."Published figure using HLA-DR monoclonal antibody (Product # 46-9952-42) in Flow Cytometry"2.Stem cell research & therapyHuman umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism."Published figure using HLA-DR monoclonal antibody (Product # 46-9952-42) in Flow Cytometry"3.Experimental and therapeutic medicineHuman umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice."Published figure using HLA-DR monoclonal antibody (Product # 46-9952-42) in Flow Cytometry"4.Proceedings of the National Academy of Sciences of the United States of AmericaRemarkably low affinity of CD4/peptide-major histocompatibility complex class II protein interactions.

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