产品介绍 抗体类型 一抗同型对照：Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-Cyanine5.5浓度：5 µL/Test用法：5 μL (0.15 μg)/test (Flow)产品详细信息Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.Applications Reported: This LN3 antibody has been reported for use in flowcytometric analysis.Applications Tested: This LN3 antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.数据HLA-DR Antibody (45-9956-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PerCP-Cyanine5.5 (Product # 45-4732-82) (open histogram) or Anti-Human HLA-DR PerCP-Cyanine5.5 (filled histogram). Cells in the lymphocyte gate were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体参考文献：1.Journal of translational medicineClinical significance of plasmacytoid dendritic cells and myeloid-derived suppressor cells in melanoma."45-9956 was used in Flow cytometry/Cell sorting to investigate multiple immune cell types in a series of blood samples from melanoma patients with different stages of the disease."2.PloS oneSoluble tumor necrosis factor related apoptosis inducing ligand level as a predictor of severity of sepsis and the risk of mortality in septic patients."45-9956 was used in Flow cytometry/Cell sorting to examine soluble TRAIL levels in septic patients to explore the association between sTRAIL level and the risk of mortality."3.OncoimmunologyCharacterization of thein vivoimmune network of IDO, tryptophan metabolism, PD-L1, andCTLA-4in circulating immune cells in melanoma."45-9956 was used in Flow cytometry/Cell sorting to demonstrate that the expression of IDO, PD-L1, and CTLA-4 in the peripheral blood of melanoma patients is strongly interconnected."4.Stem cell research & therapyComprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta."Published figure using HLA-DR monoclonal antibody (Product # 45-9956-42) in Flow Cytometry"5.Nature communicationsChallenging PD-L1 expressing cytotoxic T cells as a predictor for response to immunotherapy in melanoma."45-9956 was used in Flow cytometry/Cell sorting to study the association of PD-L1-expressing cytotoxic T cells with an altered immune climate and a negative impact on disease outcome in melanoma patients independent of treatment."

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