产品介绍 类型:人B细胞流抗同型对照：Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright 436, eBioscience浓度：5 µL/Test用法：5 μL (0.25 μg)/test(Flow)产品详细信息Description:The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This P3.6.2.8.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P3.6.2.8.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse splenocytes. Use the isotype control at the same concentration as the experimental antibody.Super Bright 436 can be excited with the violet laserline (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。未经明确授权不得转售。数据CD23 Antibody (62-0238-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (left) or Anti-Human CD23 Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体

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