产品介绍 类型: 人B细胞流抗同型对照：Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright 436, eBioscience浓度： 5μL/test用法：5μL(0.25μg)/test(Flow)产品详细信息Description:This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.Applications Reported: This IA6-2 antibody has been reported for use in flow cytometricanalysis.Applications Tested: This IA6-2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.仅用于科研。不用于诊断过程。未经明确授权不得转售。数据IgD Antibody (62-9868-42) in FlowNormal human peripheral blood cells cells were stained with CD19 Monoclonal Antibody, APC (Product # 17-0198-42) and Mouse IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4724-82) (left) or IgD Monoclonal Antibody, Super Bright 600 (right). Cells in the lymphocyte gate were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体

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