Bio-Rad 2150-0515,Native Bovine Collagen I

2024-10-22

Native Bovine Collagen I

货号:2150-0515

规格:10 mg

价格:1377

产品类型:蛋白

品牌:Bio-Rad

产品介绍
Collagen type I 90%Collagen type III 10%Non-collagenous proteins <0.5%
产品详情

Target Species

Bovine

Product Form

Purified protein from calf tendon - lyophilized

Preparation

Bovine collagen type I was isolated from calf tendons. Isolation steps included extraction into dilute acetic acidafter pepsin treatment, followed by multiple steps of differential salt precipitation and ion-exchange chromatography.

Buffer Solution

Essentially salt free

Preservative Stabilisers

None present

Protein Molecular Weight

~300 kDa

Reconstitution

Use 0.5M acetic acid, pH 3.0. Dispersed collagen have to be thoroughly homogenized in a Potter after addition of the solute and left overnight in the coldroom to dissolve.Dissolved collagen retains immunologic properties of native collagen type I. Thermal denaturation (5 minutes at 100ºC) converts dissolved collagen to gelatin.

Regulatory

For research purposes only

Guarantee

Guaranteed until date of expiry. Please see product label.
存储条件
Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use.
应用

Application Name

Verified

Min Dilution

Max Dilution

ELISA

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

技术参数

产品应用 ELISA

产品类型 Purified Protein

推荐用于 ELISA

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