产品介绍 抗体类型 一抗同型对照：Mouse IgG2a kappa Isotype Control (eBM2a), eFluor 450浓度：5 µL/Test用法：

5 μL (0.125 μg)/test (Flow)产品详细信息Description: The L243 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This L243 antibody has been reported for use in flow cytometric analysis. .Applications Tested: This L243 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chaincontains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.数据HLA-DR Antibody (48-9952-42) in FlowStaining of normal human peripheral blood cells with Mouse IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4724-82) (blue histogram) or Anti-Human HLA-DR eFluor® 450 (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.推荐产品：二抗荧光二抗免疫组化一抗标签抗体参考文献：1.Mucosal immunologyPlasmacytoid dendritic cells are scarcely represented in the human gut mucosa and are not recruited to the celiac lesion."48-9952 was used in Flow cytometry/Cell sorting to suggest that IFN-α is present in the celiac mucosa and IFN-λ is important in intestinal antiviral defense."2.Stem cell research & therapyHuman umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism."Published figure using HLA-DR monoclonal antibody (Product # 48-9952-42) in Flow Cytometry"3.Nature medicineA humanized bone marrow ossicle xenotransplantation model enables improved engraftment of healthy and leukemic human hematopoietic cells."48995242 was used in flow cytometry to develop and characterize a humanized ossicle xenotransplantation approach"4.ImmunityTissue-Resident Macrophages in Pancreatic Ductal Adenocarcinoma Originate from Embryonic Hematopoiesis and Promote Tumor Progression."48-9952 was used in Flow cytometry/Cell sorting to uncover the heterogeneity of TAM origin and functions and could provide therapeutic insight for PDAC treatment."5.Nature communicationsSingle-cell tracking of flavivirus RNA uncovers species-specific interactions with the immune system dictating disease outcome."48-9952 was used in Flow cytometry/Cell sorting to demonstrate the tracking of and sense viral RNA at single-cell resolution within complex subsets of the human and murine immune system in different mouse models."

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