ScienCellGK107人内皮素信号qPCR检测试剂盒的特点

2024-08-08

GK107 ScienCell 人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit

ScienCell 研究实验室(ScienCell)是一家生物技术公司,其使命是实验和治疗用细胞和细胞相关产品的研究和发展。 ScienCell为研究界提供各种高质量的正常的人类和动物细胞,细胞培养基和试剂,培养基添加因子,细胞来源的RNA,cDNA和蛋白质。ScienCell的科学家们以改善生活质量,实现细胞的能力,打击人类退化性疾病为目的研究和开发各种细胞治疗策略。ScienCell总部设在美国加利福尼亚州圣地亚哥,公司成立于1999年。

北京作为ScienCell品牌的特约代理商,为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。

GK107 ScienCell人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit,产品简介:

产品品牌: ScienCell

产品货号:GK107

产品名称:GK107 ScienCell人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit

产品规格:One 96-well plate, one 20ul reaction per well

GK107 ScienCell人内皮素信号qPCR检测试剂盒,Human Endothelin Signaling qPCR Array Kit, 产品说明:

ScienCell的GeneQuery™人内皮素信号传导qPCR阵列试剂盒(GQH-EDN)调查了参与内皮素信号转导的88个关键基因,特别关注内皮素-1。 内皮素是调节血管收缩的分泌蛋白,主要在内皮中产生。 内皮素受体也存在于中枢神经系统的细胞中,异常信号传导可能与癫痫发作和周围shenjingbing变有关。 与异常内皮素信号传导有关的其他疾病包括心血管疾病、血管痉挛、心脏肥大和登革热。 如何对所包含的基因进行分类的简要示例如下所示:

钙调节: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D

细胞骨架重塑:CAPN2,MAP2K1,PLD1,PLD2,RHOA

细胞周期调节:CCND3、CDC42、ITPR1、ITPR3、RIPK1

转录因子:ELK1,FOS,JUN,MYC,SP1

RAS超家族GTPases:HRAS,KRAS,NRAS,RAC1,RAP1A

GeneQuery™ qPCR 芯片试剂盒采用 96 孔板格式的 qPCR 就绪,每个孔包含一个引物组,可识别并有效扩增特定靶基因的 cDNA。精心设计的引物确保:(i)qPCR分析中的最佳退火温度为65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有说明,否则引物集可识别靶基因的所有已知转录本变体;(iii)只有一个基因被扩增。每个引物组都已通过qPCR和熔解曲线分析和凝胶电泳进行了验证。

ScienCell's GeneQuery™ Human Endothelin Signaling qPCR Array Kit (GQH-EDN) surveys 88 key genes involved in the endothelin signaling transduction with a particular focus on endothelin-1. Endothelins are secreted proteins that regulate vasoconstriction and are produced primarily in the endothelium. Endothelin receptors are also present in cells of the central nervous system and abnormal signaling may be involved in seizures and peripheral neuropathy. Other diseases implicated in aberrant endothelin signaling include cardiovascular disorders, vasospasm, cardiac hypertrophy, and Dengue fever. Brief examples of how included genes may be categorized are shown below:

· Calcium regulation: CACNA1C, CACNA1D, CALM1, CAMK2A, CAMK2D

· Cytoskeletal remodeling: CAPN2, MAP2K1, PLD1, PLD2, RHOA

· Cell cycling regulation: CCND3, CDC42, ITPR1, ITPR3, RIPK1

· Transcription factors: ELK1, FOS, JUN, MYC, SP1

· RAS superfamily GTPases: HRAS, KRAS, NRAS, RAC1, RAP1A

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人内皮素信号传导 qPCR 阵列板布局*(8 个粗体和斜体对照)

1

2

3

4

5

6

7

8

9

10

11

12

A

ADCY1

CACNA1D

CDC42

EDNRA

GSK3B

MAP2K1

MYC

PIK3R1

PPP1CA

PTGS2

RIPK1

β-actin

B

ADCY3

CALM1

CGA

EDNRB

HBEGF

MAP3K1

NOS3

PIK3R2

PPP3CC

PTK2B

SHC1

GAPDH

C

ADCY8

CALM2

CRK

EGFR

HRAS

MAPK13

NPPB

PIK3R3

PRKCA

RAC1

SOS1

LDHA

D

AKT1

CAMK2A

DGKB

ELK1

ITPR1

MAPK14

NRAS

PLA2G4A

PRKCD

RAF1

SOS2

NONO

E

AKT2

CAMK2B

DGKE

FOS

ITPR2

MAPK3

NTRK1

PLA2G7

PRKCE

RAP1A

SP1

PPIH

F

ASIC1

CAMK2D

ECE1

GNAI1

ITPR3

MAPK9

PIK3CA

PLCG1

PTGER2

RAPGEF1

SRC

GDC

G

ATF4

CAPN2

EDN1

GNAQ

JUN

MMP2

PIK3CB

PLD1

PTGER4

RASGRP1

STAT5A

PPC

H

CACNA1C

CCND3

EDN3

GRB2

KRAS

MT-CO2

PIK3CD

PLD2

PTGS1

RHOA

TRPV1

NTC

提示:基因选择可能会根据新的研究和开发进行更新

ScienCell基因表达分析产品订购信息:

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